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Engineering & Maintenance

Microbiology Division

Head of the Division

Dr. Parthiban Rudrapathy

Additional Professor
Phone - 0490-2399 283
Email - drparthiban@mcc.kerala.gov.in

The main focus of the division is to provide high-quality service and improve continuously and innovate Diagnostic Microbiology services. This also helps limit any possibility of nosocomial infections as well. Over the last decade, this division has attained important milestones. The Microbiology Division has primary responsibilities such as diagnostic laboratory services, teaching/ training and research activities. The division is equipped with diagnostic MALDI-TOF (Biomerieux), fully automated Microbial Identification and Antibiotic sensitivity testing – VITEK - 2, Automated Blood culture system BACTEC 9050 and BacT/ALERT 3D for rapid diagnosis of bacteraemia and septicaemia and fully automated ELISA (Enzyme Linked IMMUNOSORBENT Assay) immune assay system to diagnose various infectious diseases. The division looks after the diagnosis of infectious diseases complicating hematological cancers and solid tumours caused by bacteria, fungi and sero-diagnosis of viral diseases, and molecular diagnosis of viral aetiological agents in patients. It also caters to bring out guidelines for prevention of Health Care Associated Infection (HCAI), performs periodical surveillance and conducts frequent training programmes for clinicians, nursing staff and Class IV employees.

   

MCC-PGIOSR always make earnest efforts to comply with the International Standard-ISO 15189: 2012 and NABL 112 to ensure that its standard of service is of global equivalence. Accordingly, the laboratory has the following objectives:

  • To observe good clinical laboratory practices in the Pre-examination, Examination and Post examination part of testing and sample processing
  • To provide accurate, precise and reliable test results at the committed possible turnaround time
  • To adhere to the guidelines in the quality manual, quality system procedures and work instructions of the laboratory, while performing the duties and responsibilities.
  • To get the Lab. management committed to provide the required resources, support and cooperation so as to achieve the projected accuracy, precision and turnaround time
     

A) Molecular Testing
Transplant Associated Infections:

  • CMV Quantitative Real time PCR
  • EBV Quantitative Real Time PCR
  • BK Quantitative Real time PCR

HIV and Hepatitis:

  • HIV Quantitative Real-time PCR and GeneXpert
  • HCV Quantitative Real-time PCR and GeneXpert
  • HBV Quantitative Real-time PCR

Infectious Diseases:

  • COVID – 19 testing – Real-time PCR and GeneXpert
  • Drug – resistance – Real-time PCR.
  • Antibiotic resistance genes Gradient Thermal PCR.
  • Tuberculosis - GeneXpert MTB/RIF

B) Conventional culture for anaerobic bacteria
Conventional culture for anaerobic bacteria from clinical specimen other than blood is carried out on the most appropriate media in controlled environment.


C) Microbial Identification and Sensitivity testing

  • Rapid Microbial Identification – MALDI-TOF
  • Blood Culture and sensitivity - BACTEC 9050 and BacT/ALERT 3D.
  • Automated Microbial identification and susceptibility testing – VITEK 2-  compact

D) Serology

  •  Clostridium difficile toxin a & b  -  ELISA
  •  Serum galactomannan assay - ELISA
  •  Widal test
  •  ASO - Anti streptolysin O Titre
  • CRP - C-reactive protein
  • RA - Rheumatoid factor
  • RPR – Rapid Plasma Reagin

Quality control (Q.C.) and quality assurance (Q.A)

  • Quality control (Q.C.) and quality assurance (Q.A) are strictly implemented and usually authenticated by the reproducible results from other laboratories.

Hospital Infection Control activities:

The division of Microbiology is an active member of the hospital infection control committee (HICC). The division carries out surveillance of hospital-acquired infection (HAI) with the help of the infection control team. Laboratory records are analyzed to determine the rate of infection in various areas of the hospital. Presently the surveillance is passive and is based on the laboratory records of culture-positive cases. Apart from surveillance of HAI, environmental surveillance activities are also carried out which includes air sampling in critical and semi-critical areas. In addition to this, the infection control practices are also routinely monitored by the following activities

  • Sterility checks of the new pre-sterilized articles purchased by the hospital or the efficacy of the new disinfectants to be purchased is done when desired by the concerned authority.
  • In use test of the disinfectants used in the hospital Monitoring of CSSD
  • Surveillance of pathogens.
  • Infection control policies in force: disinfection, surveillance, isolation, antibiotic and chemoprophylaxis.
  • Continuing medical education (CME) programme held regularly.
     

Dr. R.PARTHIBAN
  Awarded “AVRS-India Fellow Award” by the The Antiviral Research Society (AVRS) at International Conference
on Infectious Diseases (ICID) 2023 (AVRS-ICID 2023) held on 2 December 2023 at Chemical Science Building
Auditorium, University of Madras.
  Awarded Best oral presentation award at the conference & International Conference on Infectious Diseases and
Antimicrobial Resistance (ICiDAR- 2023) in association with Indian Association of Medical Microbiologists (IAMM)
Tamilnadu Chapter on 27-29th July at chennai.
  Awarded Best oral presentation award in "Indo-Singapore International Conference on Sciences, Agriculture,
Engineering and Management (ISICSAEM 2022)- on August 13, 14 2022 (Hybrid Offline and Online)
  Awarded LOUIS PASTEUR award for the best oral presentation in the International Virtual conference on SARS-
CoV-2 CONTAGION LESSONS LEARNT&2021
  Awarded Best E-Poster in the International Virtual conference on Emerging and Re-Emerging infectious diseases
in Health care setting- MICROSUMMIT 2021

Dr. SARAVANAN M 
  Awarded best Poster presentation award at the conference on SAI MICROCON 2023 INTERNATIONAL E- CONFERENCE VIF- ARC (VIRTUAL INTELLECTUAL FRONTIER -ANTIMICROBIAL RESISTANCE AND CLINICAL MICROBIOLOGY) Date: 20th & 21st July 2023
 
 

1.    Kanadan Anjali, Muhammed Manzoor, Mangesh Vasant Suryavanshi, Parthiban Rudrapathy, Punchappady Devasya Rekha, Ranajit Das, Asif Hameed, Ananthapadmanabha Bhagwath Arun.Dysbiosis of the oral microbiota composition is associated with oral squamous cell carcinoma and the impact of radiotherapy: A pilot study. FEMS Microbiology Letters, Volume 370, 2023 
2.    Saravanan M, Sajani Samuel, Sarath KE, Parthiban R. Retrospective investigation of antibiotic resistance pattern by Microbial pathogens isolated in surgical site infections in cancer patients. Research Journal of Pharmacy and Technology, Vol: 16, Issue: 10 (2023) 
3.    Sajani Samuel, Vismaya, Parthiban R, Saravanan M, and Sarath K.E. ESBL Prevalence in Urine Specimens of Cancer Patients at a Tertiary Care Cancer Centre in Kerala -  Asian Journal of Pharmaceutical and clinical research, Vol 16 Issue 6, (2023). 
4.    Nirmal G, Jithin TK, Gopakumar KG, Parthiban R, Nair C. Prevalence and Outcomes of Carbapenem-resistant Bloodstream Infection in Children With Cancer. J Pediatr Hematol Oncol. 2023 Aug 1;45(6):e678-e682. 
5.    Parthiban Rudrapathy, Shythya K, Sajani Samuel ,Sarath KE , Saravanan Murugesan Molecular characterisation of Carbapenem resistant Gram Negative Bacilli in a tertiary care cancer centre. Int J Pharm Bio Sci,.13(1),B 83-89.2022 
6.    Parthiban Rudrapathy,Sujina TK, Sajani Samuel, Sarath KE, Saravanan Murugesan. Microbiological profile and Antibiotic resistance of bloodstream infections among cancer patients at a tertiary care cancer centre in North Kerala, India.  National Journal of Laboratory Medicine.2022.Jan, Vol-11(1):  MO16-MO20 
7.    Sajani Samuel, Neeraja.O, Parthiban R, Saravanan M, and Sarath K.E. Distribution of resistant genes encoding extended-spectrum beta –lactamases in enterobactericeae isolated from various clinical samples in a tertiary care       cancer centre. (2022). NJLM - National Journal of Laboratory Medicine.11(2):1-5
8.    Dilsha TK, Sajani Samuel, Parthiban Rudrapathy, Saravanan Murugesan, Sarath KE. Antibiotic resistance, meca gene detection, and biofilm formation ability among coagulase-negative staphylococci in cancer patients. Asian J Pharm Clin Res, Vol 15, Issue 1, 2022, 35-39. 
9.    Parthiban Rudrapathy, Athira KV, Sajani Samuel and Saravanan Murugesan. Phenotypic Detection of Metallo- Beta-Lactamase (MBL) Producing Gram-Negative Bacteria and Their Prevalence in a Tertiary Care Cancer Centre. Int J Pharm Bio Sci,.12(3),56-62.2021 .
10.    Saravanan Murugesan, Sujina TK, Sajani Samuel, Sarath KE, Parthiban Rudrapathy. Methicillin-resistant staphylococci prevalence in cancer patients at a tertiary care cancer centre – a retrospective study. Asian Journal of Pharmaceutical and Clinical Research.  Vol 14, Issue 7.41482.2021 
11.    Sajani Samuel, Parthiban R, Saravanan M, Sarath K.E .Changing Trends in Antimicrobial Resistance among Gram-negative Bacilli isolated from Respiratory tract of Cancer Patients at a Tertiary Cancer Centre: A 4-year Retrospective Study. Int J Pharm Bio Sci,2021.12.2.b114-123.
12.    Chandran K.Nair aManuprasad Avaronnan aPraveen Kumar Shenoya Vineetha RaghavanaPriya Jayarajan, bParthiban RudrapathycKarthickeyanDuraisamyd Satheesan balasubramaniane.Impact of active tuberculosis on treatment decisions in cancer. Current Problems in Cancer.https://doi.org/10.1016/j.currproblcancer.2020.10064
13.    Saravanan Murugesan, Ph.D, Sajani Samuel, Ph.D, and Parthiban Rudrapathy, Ph.D. Microbiological Profile And Antibiotic Susceptibility Pattern Of Uropathogens Isolated Among Cancer Patients At A Tertiary Care Cancer Centre, South India. Int J Pharm Bio Sci Volume 11 Issue 2, 2020 (April-June), Pages:56-63 
14.    K Anjali, A B Arun, T S Bastian, R Parthiban,M Selvamani, H Adarsh. Oral microbial profile in oral cancer patients before and after radiation therapy in a cancer care center –A prospective study.Journal of Oral and Maxillofacial Pathology,2020. 
15.    Anjali Kanadan1, ArunAnatapadma Bhagwath2, BastitianThattil Sebastian3, Parthiban Rudrapathy4,Selvamani Manickam3.Study on oral microbial flora and antibiotic sensitivity pattern among oral cancer patients ina tertiary cancer care center. Journal of Orofacial Sciences11[2] 2019. 
16.    Sajani Samuel, Gufran Ahmed, Parthiban Rudrapathy, Saravanan Murugesan. Prevalence and Resistance Pattern of Moraxella catarrhalis causing Respiratory Tract Infections in Cancer Patients.Online Journal of Health and Allied Sciences 18[1]2019. 
17.    Sajani Samuel, Safeera A, Gufran Ahmed, Parthiban Rudrapathy, Saravanan Murugesan, Emergence of Non- Diphtheritic Corynebacterium as a Co- Pathogen in Various Clinical Specimens in Cancer Patients. Online Journal of Health and Allied Sciences18[1]2019 .
18.    Saravanan Murugesan*, Parthiban Rudrapathy, Sajani Samuel. A case of pediatric leukemia with multi drug resistant bacterial infection. International Journal of Contemporary Pediatrics 2019.
19.    Saravanan Murugesan , Parthiban Rudrapathy,Sajani Samuel. Emergence of Unusual Gram-Negative Bacteria Among Cancer Patients in a Tertiary Care Cancer Centre at North Kerala. Online Journal of Health and Allied Sciences 17[4]2019.
20.    Sajani Samuel, Gufran Ahmed, Parthiban Rudrapathy, Saravanan Murugesan. Microbial profileand antimicrobial susceptibility of gram negative aerobic bacteria causing respiratory tract infections in cancer patients. Indo – Asian Journal of Multidisciplinary Research 4[5] 2018.

S. No

Existing Facilities

Purpose

1

VITEK - 2

Automated Bacterial Identification and Susceptibility testing

2

VITEK-MS (MALDI-TOF)

Automated Microorganisms Identification (Rapid)

3

BAcT/ALERT -3D

Automated Blood culture System

4

Bactec – 9050

Automated Blood culture System

5

Real-Time PCR

Molecular diagnosis for Infectious diseases

6

GenXpert

Molecular diagnosis for Infectious diseases

7

Anaerobic Chamber

Culture of Anaerobic Microroganisms

8

Thermofisher- KingFisher Flex System

Automated Extraction system for RNA/DNA

9

Refrigerated Centrifuge

Used for RNA/DNA Extraction

10

Fluorescence Microscope

For Mycobacterium tuberculosis identification

11

Gradient Thermal Cycler

For Research Purpose

1. M.Sc Medical Microbiology (Affiliated under KUHS university)

2. Certificate course on " Molecular Techniques in Clinical Applications ( ASAP & MCC- PGIOSR certified )

Academic activities:

1. Classes for B.Sc MRT & Nursing students.

2. Projects undertaken for Postgraduate & PhD Students.

3. Induction training classes .

CME conducted:
 1. CME ON " Updates in Microbiology and Immmunology  - 24th November 2018